The experimental process is quite a mess. I’ve been
realizing more and more that science is imperfect and more often than not
things go wrong. Unfortunately this was proven true for our Mod2 experiment. There was little to no statistical significance in the findings and this will make
going home for Thanksgiving interesing since they’ll be asking what I’ve done
and my response will be 'well I failed to upregulate Lactate production by using
CRISPRi technology to plant a ‘roadblock’ to prevent transcription, and
therefore function of the enzyme pflB to try and increase pyruvate to funnel
more of that product into Lactate. And since none of the other words really
make any sense they’ll just hear I failed.
It
sounds kinda sad but honestly I’m just excited to be doing real science
and I wish we had time to go back and try another guide or run more trials and
try and find out what went wrong since these implications are really powerful
and I wish we had found something more substantial. I’m glad we got an
introduction into CRISPR technology and having more of an idea of what it is
amazing since it has so many possible applications. Writing the Mod2 report has been a great way to synthesize all that we learned and it opened my eyes to
all the places the project could have gone wrong and so I think it was a great
way to end the module! Thank you, instructors, for all you do!
No comments:
Post a Comment